Journal: iScience

Article Title: cAMP-related second messenger pathways modulate hearing function in Aedes aegypti mosquitoes

doi: 10.1016/j.isci.2025.113202

Figure Lengend Snippet: Octopamine potentially overlaps with presynaptic sites in male and female Ae. aegypti flagellar ears (A) Schematic diagrams of male (left panel) and female (right panel) ears and interpretation of different ear parts: for each panel, flagellum (top left), fibrillae (top right), pedicel (bottom, left) and JO (bottom right). Red and orange represent JO and flagellar neurons in the ear, respectively. Yellow and cyan dots represent octopamine and presynaptic sites, respectively. Ax, axons of JO neurons and efferent neurons; BP, basal plate; D, dendrites of JO neurons; P, prong; SO, somata array of JO neurons. A, anterior; M, medial. (B) Location of presynaptic sites in male (top row) and female (bottom row) ears. Sections of male JO (left), JO close-up (middle) and flagellum (right) are shown. Anti-horseradish peroxidase (HRP; red) used as neuronal marker, anti-SYNORF1 (3C11; cyan) labels presynaptic sites. White line selected neuron axons. Scale bar = 20 μm. (C) Location of octopamine in male (top row) and female (bottom row) ears. Sections of male JO (left), JO close-up (middle) and flagellum (right). Anti-HRP (red) used as neuronal marker, anti-octopamine (OA, yellow) shows octopamine localization. DAPI (blue) was used for flagellum. FL, flagellum. White line, selected neuron axons. White square, selected octopamine signals in the brain. Scale bar = 20 μm. See also .

Article Snippet: Mouse monoclonal anti-SYNORF1 3C11 , Developmental Studies Hybridoma Bank , RRID: AB_528479.

Techniques: Marker